RNF149 Knockout cell line (HeLa)

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location： Home > Products > KO Cell Lines > RNF149 Knockout cell line (HeLa)

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RNF149 Knockout cell line (HeLa) Order

RNF149 Knockout cell line (HeLa)
Catalog#
YKO-XN92920
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RNF149 Knockout cell line (HeLa)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RNF149 Knockout cell line (HeLa)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
RNF149
Gene ID
284996
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 1 (based on transcript RNF149-201)
Validation: PCR+Sanger Sequencing
RNF149 Knockout cell line (HeLa) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HeLa		 STR Info (Cell bank)
Cell Line:HELA
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 1213.31213.3
D7S820 812812
D16S539 910910
VWA 16181618
TH01 7777
AMEL XXXX
TPOX 812812
CSF1PO 910910
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RNF149 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HeLa cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RNF149 gene knockout RNF149 Knockout cell line (HeLa) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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